Abstract
Expression of paternal alleles for 20 loci expressed during early seed development in Arabidopsis was undetectable during early embryo and endosperm development, suggesting the presence of imprinting of the paternal genome.
Highlights
Studies in embryogenesis and seed development in plants have a long history, yet the underlying mechanism for the transition from the maternal to the zygotic phase, along with many other events that follow to make an embryo in the seed, remains largely unknown
When wildtype pollen was crossed to the transposant lines, all of the progeny showed GUS expression patterns identical to the self-fertilized transposant lines
To confirm that the GUS expression pattern reflected the expression of endogenous genes, in situ hybridization was performed using an RNA probe specific for a gene tagged by one of the Ds elements in one transposant line
Summary
Studies in embryogenesis and seed development in plants have a long history, yet the underlying mechanism for the transition from the maternal (haploid egg) to the zygotic (diploid sporophyte) phase, along with many other events that follow to make an embryo in the seed, remains largely unknown. To screen for loci that are expressed during early ovule and seed development, Vielle-Calzada et al used enhancer-detector lines that contain Ds mobile elements linked to a uidA gene, which encodes αglucuronidase (GUS). They identified 19 lines that show GUS expression in the developing embryo and/ or endosperm tissue after fertilization.
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