Abstract
AbstractNovelty: A fowl pox virus vector is described which will allow the insertion and expression of foreign DNA sequences as a method of producing a live viral vaccine carrier.Biology: The fowl pox virus genome is 320–380 kb and is similar in structure to vaccinia virus. The insertion of a vaccinia virus thymidine kinase gene and an E. coli lac Z gene in a non-essential site is described and this allows the insertion of any other foreign DNA fragment into the recombinant virus, provided it contains a vaccinia virus tk gene flanking regions for in vivo recombination. Also described is the culture of chicken embryo fibroblast cell lines and chicken embryo dermal cells for the in vitro production of foreign proteins using the novel fowl pox vectors. A large list of foreign proteins, such as various blood clotting factors and HIV antigens, are described as proteins which could be expressed using this system.
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