Abstract
Past and Present of Reverse Genetics in Animal Virology with Special Reference to Non-Segmented Negative Stranded RNA Viruses: a Review
Highlights
The paradigm of “genetics” is usually explained as phenotype to genotype, and this has led to the idea of genotype to phenotype being called as “reverse genetics”
Earlier works on generation of recombinant virus were concentrated in DNA virus, in which the naked DNA was transfected into permissive cell lines to rescue the progeny virus
The rescue of progeny virus from cDNA was established for positive sense RNA (PS–RNA)
Summary
The paradigm of “genetics” is usually explained as phenotype to genotype, and this has led to the idea of genotype to phenotype being called as “reverse genetics”. Reverse Genetics of NSNS RNA Viruses Different Strategies Adopted to Rescue NSNS RNA Virus from cDNA The technique of manipulation at the genome level with the aid of helper plasmids has increased the scope for rescuing other viruses with non–segmented negative sense single stranded RNA genome like Newcastle disease virus.
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