Passively propagated spikes from the soma of cells in pineal gland of guinea pigs.

Abstract

The membrane properties of cells within the pineal gland of guinea pigs were studied using intracellular electrophysiological techniques. The electrotonic responses to intracellular current injection decayed with a single exponential in approximately 60% of cells but was preceded by a quicker component in the remainder. The membrane time constant was 2.8 ms. Depolarization beyond -29 mV activated an outward current that reversed at around the value of the resting potential. Hyperpolarization activated a slow inward current. Spikes occurred in response to activation of alpha-adrenoceptors. They were resistant to tetrodotoxin but were abolished by nifedipine and verapamil, suggesting that calcium carries the current during their upstroke. Spikes could not be evoked by depolarizing current pulses of 1-ms to 2-s duration. The responses to hyperpolarizing current steps or voltage-clamp steps applied during the peak of spikes evoked by nerve stimulation were indistinguishable from the responses to those applied between spikes. During nerve stimulation, fluctuations were observed in the current trace of cells under voltage clamp, indicating that the spikes could not be voltage clamped successfully. It is concluded that the spikes occurring in response to nerve stimulation are generated on the processes of the pinealocytes and are passive in the soma.