Abstract
The results obtained in this study indicate that delayed hypersensitivity to old tuberculin (OT) can be passively transferred from BCG-vaccinated to normal guinea pigs with cells or cellular extracts. Treatment of sensitive cells with purified protein derivative (PPD) in vitro induced the release of the transfer material from intact cells; PPD-treated cells lost, and the surrounding medium gained the property of transferring delayed hypersensitivity. It was found that the transfer material-depleted cells can regain the ability to transfer the hypersensitivity after incubation for a few days in tissue culture medium. Also the release of the transfer material from cells into blood can be accomplished in vivo by treatment of hypersensitive animals with a strong dose of OT. Such OT challenge renders the hypersensitive animal temporarily desensitized to subsequent tuberculin tests. Heat sensitivity, dialysis, and passive serum transfer experiments indicate that the characteristics of the transfer material do not correspond with those of a conventional antibody. The limited duration of hypersensitivity in passively sensitized animals suggests that the material cannot be replicated in the body of the normal animal.
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