Abstract
This study was conducted to evaluate the effect of extracts of Passiflora edulis Sims leaves on the oxidative metabolism of rat peritoneal neutrophils using a model of acute inflammation. The extract was obtained by maceration in 70% ethanol, evaporation under reduced pressure and lyophilisation. Total phenolic content (TP) was determined by the Folin-Ciocalteu assay. The P. edulis extract, in different doses, was administered by gavage 1 h prior to inflammation induction by carrageenan (8 mg/kg, i.p.); five hours later, the neutrophils were obtained by intraperitoneal lavage. The tests performed in neutrophils were cytochrome C and chemiluminescence assay as well as myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities. The administration of the extract reduced the number of neutrophils recruited to the site of inflammation; however, the extract did not alter the activity of NADPH oxidase as well as SOD activity in these cells. The MPO and CAT activities in peritoneal neutrophils of rat treated with extract was lower than in the control group, and the GPx activity was increased. Based on the experimental model utilised, the anti-inflammatory potential of P. edulis leaf extract could be related to the presence of phenolic compounds in the extract.
Highlights
Neutrophils are recruited at the onset of the pathogen defence response as an essential component of the acute inflammatory process
With regard to effects on antioxidant enzymes, no significant differences in the superoxide dismutase (SOD) activity were observed in peritoneal neutrophils from groups treated with the P. edulis extract compared to the C group (Figure 6A)
This study evaluated the anti-inflammatory potential of P. edulis leaf extract by employing a model of acute peritonitis, which was induced by the administration of carrageenan
Summary
Neutrophils are recruited at the onset of the pathogen defence response as an essential component of the acute inflammatory process. Despite the regulation of ROS production and use, this oxidative metabolism can set off an ongoing inflammatory process, leading to an oxidative stress status that can cause serious damage to the host organism. In such cases, endogenous antioxidants are often unable to prevent oxidative damage and require exogenous scavengers that. Considering that oxidative stress has been linked to the development of several diseases (Carocho, Ferreira, 2013), this study evaluated the effects of P. edulis leaf extract on the oxidative metabolic processes in rat peritoneal neutrophils by assessing ROS production and the activity of antioxidant enzymes of these cells
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