Abstract
The antifibrotic effects of traditional medicinal herb Caesalpinia sappan (CS) extract on liver fibrosis induced by thioacetamide (TAA) and the expression of transforming growth factor β1 (TGF-β1), α-smooth muscle actin (αSMA), and proliferating cell nuclear antigen (PCNA) in rats were studied. A computer-aided prediction of antioxidant and hepatoprotective activities was primarily performed with the Prediction Activity Spectra of the Substance (PASS) Program. Liver fibrosis was induced in male Sprague Dawley rats by TAA administration (0.03% w/v) in drinking water for a period of 12 weeks. Rats were divided into seven groups: control, TAA, Silymarin (SY), and CS 300 mg/kg body weight and 100 mg/kg groups. The effect of CS on liver fibrogenesis was determined by Masson's trichrome staining, immunohistochemical analysis, and western blotting. In vivo determination of hepatic antioxidant activities, cytochrome P450 2E1 (CYP2E1), and matrix metalloproteinases (MPPS) was employed. CS treatment had significantly increased hepatic antioxidant enzymes activity in the TAA-treated rats. Liver fibrosis was greatly alleviated in rats when treated with CS extract. CS treatment was noted to normalize the expression of TGF-β1, αSMA, PCNA, MMPs, and TIMP1 proteins. PASS-predicted plant activity could efficiently guide in selecting a promising pharmaceutical lead with high accuracy and required antioxidant and hepatoprotective properties.
Highlights
An institutional investigation by the University of Malaya found there was no system to index and file data and images to avoid mislabeling and mishandling, which led to errors and duplication of research data
The antifibrotic effects of traditional medicinal herb Caesalpinia sappan (CS) extract on liver fibrosis induced by thioacetamide (TAA) and the expression of transforming growth factor β1 (TGF-β1), α-smooth muscle actin, and proliferating cell nuclear antigen (PCNA) in rats were studied
Rchalcone with pa 92% compared to the other constituents
Summary
An institutional investigation by the University of Malaya found there was no system to index and file data and images to avoid mislabeling and mishandling, which led to errors and duplication of research data. The authors said that both images (1(c) and 1(d)) in Figure 1 in [1] are correct and the same as illustrated in the thesis by Kadir [3]. Panel (c) in both figures represented the same reference group (silymarin group-SY) because they ran two experiments in rats treated with different plants, but with the same reference group This is illustrated in Kadir’s thesis [3]. This is illustrated in the thesis [3]. A. Kadir, Hepatoprotective role of vitex negundo and caesalpinia sappan in thioacetamide-induced liver injury in rats [Ph.D. thesis], University Malaya, 2014. A computer-aided prediction of antioxidant and hepatoprotective activities was primarily
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