Abstract
A comprehensive characterization of the humoral response towards a specific antigen requires quantification of the B-cell receptor repertoire by next-generation sequencing (BCR-Seq), as well as the analysis of serum antibodies against this antigen, using proteomics. The proteomic analysis is challenging since it necessitates the mapping of antigen-specific peptides to individual B-cell clones. The PASA web server provides a robust computational platform for the analysis and integration of data obtained from proteomics of serum antibodies. PASA maps peptides derived from antibodies raised against a specific antigen to corresponding antibody sequences. It then analyzes and integrates proteomics and BCR-Seq data, thus providing a comprehensive characterization of the humoral response. The PASA web server is freely available at https://pasa.tau.ac.il and open to all users without a login requirement.
Highlights
To provide accessibility to non-expert users we introduce the PASA (Proteomic Analysis of Serum Antibodies) web server that provides a robust computational platform for the analysis and integration of data obtained from proteomics of serum antibodies and enables its integration with BCRSeq data as obtained from ASAP
The hallmark of the adaptive immune response is based on its ability to generate an enormous diversity of different monoclonal antibodies mainly by chromosomal rearrangement, somatic hypermutation (SHM), and class-switch recombination
Next-generation sequencing (NGS) of B cell receptors (BCR-Seq) revolutionized our ability to capture the diversity of antibodies at the highest resolution and helped address important immunological questions related to the development of the adaptive immune response in health and disease [4,5]
Summary
Citation: Avram O, Kigel A, Vaisman-Mentesh A, Kligsberg S, Rosenstein S, Dror Y, et al (2021) PASA: Proteomic analysis of serum antibodies web server. PLoS Comput Biol 17(1): e1008607. https://doi.org/10.1371/journal.pcbi.1008607 A comprehensive characterization of the humoral response towards a specific antigen requires quantification of the B-cell receptor repertoire by next-generation sequencing (BCR-Seq), as well as the analysis of serum antibodies against this antigen, using proteomics. The proteomic analysis is challenging since it necessitates the mapping of antigenspecific peptides to individual B-cell clones.
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