Partly fertile interspecific hybrid between a black gram × green gram derivative and an adzuki bean

Abstract

AbstractA true‐breeding black gram (Vigna mungo) × green gram (Vigna radiata) derivative was reciprocally crossed with adzuki bean, Vigna angularis. Pollinated pistils were treated with gibberellic acid (GA3) (70 p.p.m.) 24 and 48 h after pollination. One fertile pod containing two hybrid seeds was obtained, when V. angularis was used as the male parent. One of these seeds germinated and a partly fertile interspecific hybrid was obtained. Its hybridity was confirmed at the seedling stage based on the presence of the green epicotyl colour of the adzuki bean. Pubescence of epicotyl, stem, leaf margins and pod, and the colour of the mature pod, characteristics of the black gram × green gram derivative, were also expressed in the hybrid. In addition, the hybrid exhibited two novel traits — the presence of racemose inflorescence and plant regeneration ability — not present in the parents. The study of this hybrid and four offspring revealed that green epicotyl colour of adzuki bean, and the pubescence of stem and leaf margins of the black gram × green gram derivatives were dominantly inherited traits. Colours of epicotyl and stem bases were found to be linked.