Abstract
The partition behaviour of Fursarium solani pisi recombinant cutinase was studied in polyethylene glycol (PEG)-aqueous sodium or potassium phosphate solution biphasic systems. The partition coefficient of cutinase was enhanced with decreasing molecular mass of the PEG and increasing tie-line length and pH, and was also influenced by the type of cation (Na + or K +) present in the system. Particular attention was paid to the influence of pH and the type of cation on the phase diagrams of PEG-phosphate systems.
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