Abstract
The composition of the emulsified oil and of the micellar phases obtained when a glyceride-fatty acid mixture is dispersed in bile salt solution has been defined. The micellar phase in equilibrium with the emulsified oil phase was obtained by filtration through Millipore filters. The behavior of different lipids in such systems was defined as the partition ratio, micellar/emulsified oil phase (m/o). Partition of fatty acids was found to be strongly dependent on the chain length of the fatty acid and the pH of the dispersion. The curve for partition against pH for oleic acid was interpreted to show a pK(a) for oleic acid in bile salt solution of approximately 7. The partition between micellar and oil phases is given for a series of lipids of different polarity. No significant difference in behavior was found for cholesterol and sitosterol. A relationship was found between the partition m/o and filtration rates through a Millipore filter in micellar solution. The lower the partition coefficient the lower was the rate of filtration. The results obtained are discussed in relation to the mechanism of absorption of fat from the small intestine.
Highlights
Partition of fatty acids was found to be strongly dependent on the chain length of the fatty acid and the pH of the dispersion
Evidence at hand indicates that absorption of lipids takes place mainly from the micellar phase (2) and that this micellar phase is continuously generated from the emulsified oil phase by reactions catalyzed by pancreatic lipase (3)
The emulsion obtained was diluted tenfold with bile salt solution, and 5 ml was transferred to one side of filtration chambers that contained Millipore filters of different pore sizes
Summary
The micellar phases were separated from the bile salt dispersions in filtration chambers designed according to Ogston and Phelps (11) with slight modification. The emulsions were prepared either by sonication for 1 min in a Branson sonicator (Branson Instruments, Inc., Stamford, Conn.) at maximum intensity in a volume of [5,6,7,8,9,10] ml, or by vigorously shaking by hand the glyceride mixture with the bile salt solution in a stoppered test tube, or with the aid of a Super-Mixer (Lab-Line Instruments, Inc., Melrose Park, Ill.) for about 30 sec The effect of these different procedures will he discussed later. Shaking by hand or with a Super-Mixer for [30-60] sec produced an
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
