Abstract
AbstractThe chromatographic behavior of eight lanostanoid triterpenes, which are four pairs of C‐3 epimers and two pairs of C‐3/C‐15 positional isomers, were determined by reverse‐phase high performance liquid chromatography (RP‐HPLC). In the mobile phase systems of acetonitrile‐water‐acetic acid, methanol‐water‐acetic acid, and tetrahydrofuran‐water‐acetic acid, these triterpenes showed a very good linear relationship between capacity factors (k′) and volume fractions of organic modifiers. The partition coefficients (Poet) of these triterpenes in 1‐octanol/water and capacity factors (k′) in RP‐HPLC, when both expressed in logarithm, correlated linearly. This study showed that RP‐HPLC is an effective method to evaluate the molecular hydrophobicity of multi‐functional compounds which are stereo‐ and positional isomers.
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