Abstract
An improved technique for particle analysis by disk centrifugation utilizing an externally produced density gradient and with corrections for the wavelength and angular distribution of the transmitted light has been developed. The sedimentation constant is calculated from the radial dependence of spin fluid viscosity and density. Light scattering corrections are calculated by means of calibration curves, representing the efficiency of light scattering as a function of size and relative refractive index of particles. Calibration curves for the white light in the photosedimentometer are obtained by integration across the wavelength distribution of the lamp, the sensitivity distribution of the photo diode and the angular distribution of the forward scattered light obtained from Mie theory. Coefficients of variation below 2% may be calculated using different spin fluid volumes to estimate the instrument variance.
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