Abstract
Fusarium graminearum is a major global pathogen of cereals and is considered the main causal agent of Fusarium head blight disease in wheat. Infection with Fusarium graminearum causes a significant reduction in crop yield and quality; therefore, it is very important to improve wheat pathogen resistance. In the present study, the plasmid pAHCht-2 harboring the rice chitinase (Cht-2) gene for pathogen resistance and the plasmid pAB6 containing the gus reporter and bar selectable marker genes were used for genetic transformation of immature embryo-derived calli of the Egyptian wheat cultivar Giza 164 using particle bombardment. Associated changes in defense mechanisms in the transgenic plants were investigated. The transgenic plants had significantly decreased total protein content, phenolic compounds and antioxidant enzyme activities (peroxidase and catalase), and significantly increased phenylalanine ammonia lyase and chitinase activities compared with non-transgenic plants under biotic stress conditions caused by F. graminearum infection. Our results show that activating a specific program of gene expression related to environmental stress conditions can reduce the cost of the stress on plant metabolism.
Highlights
Experimental features the plasmid pAHCht-2 harboring the rice chitinase (Cht-2) gene for pathogen resistance and the plasmid pAB6 containing the gus reporter and bar selectable marker genes were used for genetic transformation of immature embryo-derived calli of the Egyptian wheat cultivar Giza 164 using particle bombardment
The objective of the present work was to produce pathogen-resistant wheat transformed with a rice chitinase gene (Cht-2) and to detect the associated changes in defense mechanisms in transgenic plants infected with Fusarium graminearum compared with non-transgenic plants
The objective of the present work was to produce pathogen-resistant wheat plants (Triticum aestivum L. cv. 164) transformed with a rice chitinase gene (Cht-2) and to detect the associated changes in defense mechanisms in transgenic plants infected with Fusarium graminearum compared with non-transgenic plants
Summary
The objective of the present work was to produce pathogen-resistant wheat transformed with a rice chitinase gene (Cht-2) and to detect the associated changes in defense mechanisms in transgenic plants infected with Fusarium graminearum compared with non-transgenic plants. We succeed to produce a genetically transformed wheat plants with increased pathogen resistant. The results confirmed that genetic transformation of wheat plants with a rice chitinase gene (Cht-2) caused significant physiological changes including increases in chitinase and PAL activities compared with nontransgenic plants. The objective of the present work was to produce pathogen-resistant wheat plants 164) transformed with a rice chitinase gene (Cht-2) and to detect the associated changes in defense mechanisms in transgenic plants infected with Fusarium graminearum compared with non-transgenic plants The objective of the present work was to produce pathogen-resistant wheat plants (Triticum aestivum L. cv. 164) transformed with a rice chitinase gene (Cht-2) and to detect the associated changes in defense mechanisms in transgenic plants infected with Fusarium graminearum compared with non-transgenic plants
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