Abstract
In this study, we investigated the effects of preincubation with the reactive oxygen species-generating system xanthine oxidase/xanthine on Ca2+-dependent potassium permeability of erythrocyte membrane. The increase of intracellular calcium concentration in presence of calcium ionophore A23187 led to erythrocyte membrane hyperpolarization due to opening of Ca2+-activated potassium channels. Erythrocyte membrane potential was recorded via measurement of pH of the incubation medium in presence of prothonophore. Incubation of erythrocytes with xanthine (100 µmol)/ xanthine oxidase (10 mU/ml) mixture resulted in significant loss of amplitude and rate of hyperpolarization response and also loss the rate of membrane potential restoration. These effects can be caused by hydrogen peroxide, one of products of reaction of xanthine oxidase/xanthine.
Highlights
We investigated the effects of preincubation with the reactive oxygen species-generating system xanthine oxidase/xanthine on Ca2+-dependent potassium permeability of erythrocyte membrane
The increase of intracellular calcium concentration in presence of calcium ionophore A23187 led to erythrocyte membrane hyperpolarization due to opening of Ca2+-activated potassium channels
Erythrocyte membrane potential was recorded via measurement of pH of the incubation medium in presence of prothonophore
Summary
We investigated the effects of preincubation with the reactive oxygen species-generating system xanthine oxidase/xanthine on Ca2+-dependent potassium permeability of erythrocyte membrane. Амплитуда ГО и скорость его развития характеризуют Са2+-активируемые калиевые каналы, а скорость восстановления МП — активность Са2+АТФ-азы [6] Для исследования Са2+-активируемых калиевых каналов был применен метод регистрации мембранного потенциала в суспензии эритроцитов по изменениям рН среды инкубации в присутствии протонофора, основанный на том, что в этих условиях распределение протонов зависит от мембранного потенциала [6].
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