Abstract
Previous studies showed that murine polymorphonuclear leukocytes (PMNs) lyze tumour cells in the presence of wheat germ agglutinin or actinomycin D. This paper reports studies on whether a soluble factor participates in PMN-mediated cytolysis dependent on lectin or a chemotherapeutic drug. Tumour lysis was observed with supernatants from PMN cocultured with wheat germ agglutinin or actinomycin D. The supernatant from cultures of PMNs alone was not cytotoxic, but addition of these agents to the supernatant induced tumour lysis. PMNs released a soluble factor spontaneously into the medium and cytolysis was induced by a combination of this factor and wheat germ agglutinin or actinomycin D. This factor was not an oxygen metabolite, but a protein with a molecular weight of approximately 100 K daltons. These results suggest that a soluble factor(s) from PMNs participates in tumour killing in cooperation with appropriate reagents.
Highlights
We investigated the mechanisms of Polymorphonuclear leukocytes (PMNs)-mediated cytolysis dependent on lectin and chemotherapeutic drug, by studies on whether soluble factor from PMNs participates in tumour killing in vitro
We found that a PMN-derived factor can lyse tumour cells in cooperation with wheat germ agglutinin or actinomycin D and that this factor is a protein of high molecular weight
Cytolytic activity of supernatants of cultures of PMNs We used Marbrook vessels to study the role cell-tocell interaction in cytolysis, i.e. whether effectortarget cell interaction is necessary for cytolysis
Summary
Inbred male C3H/He and DDY mice were obtained. Received 22 April 1985; in revised form 22 June 1985. From Shizuoka Experimental Animal Farm (Shizuoka, Japan). Mice were used at 8-11 weeks of age. MM46, a transplatable ascites tumour from a spontaneous mammary adenocarcinoma in a C3H/He mouse, was mainly used as a target cell. MM48 mammary adenocarcinoma and MH 134 hepatoma cells were used as target cells. L929 cells were harvested from in vitro culture
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
