Partial sequences of two genes regulated by amino acid supply identified by the use of RNA fingerprinting by arbitrarily primed PCR

Abstract

RNA fingerprinting by random arbitrary primed PCR (RAP-PCR) using arbitrary 10 mer oligonucleotide primers was used to obtain partial sequences of two growth sensitive genes expressed in F9 embryonal carcinoma cells. The genes have been designated E800 and E550. The steady state levels of E800 and E550 mRNA increase when cells are cultured in lysine deficient medium. In F9 cells the pattern of E800 and E550 expression is similar to that of the growth arrest specific gene gas5. The partial sequence of E800 shows significant sequence identity to mouse and human expressed sequence tags. The deduced protein sequence shows some similarity to the ribosomal protein S7. The partial sequence of E550 shows no significant similarity with any entry in the database. In adult mice E800 is expressed in brain heart kidney adipose tissue and skeletal muscle and has a similar pattern of expression to the growth arrest gene gas5 in differentiated tissue. Our data suggest that the E800 and gas5 genes may share a common regulatory mechanism.