Abstract
Microscopic organisms play a vital role in the field of green technology. Among the genera fungi, the group of entomopathogens includes Beauveria bassiana, which has been found to have multifunctional applications. In this present research, we have attempted to partially purify chitinase from two strains of Beauveria bassiana (F881 and F911), each cultured in minimal media with 1% (w/v) colloidal chitin as well as in Sabouraud's dextrose media with 1% (w/v) colloidal chitin. The purification process was done with a setup of ceramic membranes, which comprises two stages, ultrafiltration (50 nm pore size membrane) and nanofiltration (MWCO 5 kDa). Furthermore, with the analysis of yield of enzymatically hydrolyzed NAG (N-acetyl glucosamine), resistance and fouling of the membrane were also studied.
Highlights
IntroductionThe group of entomopathogens includes Beauveria bassiana, which has been found to have multifunctional applications
Microscopic organisms play a vital role in the field of green technology
When Beauveria bassiana comes in contact with the insect’s cuticle, it releases chitinase and breaks the polymeric chain of chitin into N-acetyl glucosamine (GlcNAc) monomeric units
Summary
The group of entomopathogens includes Beauveria bassiana, which has been found to have multifunctional applications. The chitin-hydrolyzing enzyme, chitinase, has gained interest in different biotechnological applications and in food technology (KARTHIK et al, 2014). It is a white and sweet powder with a melting point of 221 °C. Driving forces may effectively enhance membrane separation processes based upon the selective permeability of one or more components of the liquid In such cases, the separation (ultrafiltration, microfiltration, and nanofiltration) relies on mass transport, which depends on the size of the components. An attempt was made towards chitinase purification from a microbial source, purification of filamentous fungal chitinase, through membrane filtration process, with crossflow ceramic membrane used in ultra- and nanofiltration
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