Partial rat kidney resection using autologous fibrinogen thrombin adhesive system.

Abstract

The Fibrinogen Thrombin Adhesive System (FTAS) enables local haemostasis to occur in parenchymatous organs without tissue damage. The aim of this study was to investigate the degradation of FTAS and the process of wound healing after partial kidney resection in rats using FTAS for induction of local haemostasis. In 28 rats partial kidney resection was performed bilaterally. Haemostasis was achieved with Fibrinogen Thrombin Adhesive System. Four experimental groups were formed. Group A (n = 3): Haemostasis with unlabelled FTAS, subcutaneous injection of 0.1 ml = 60 mu Ci Na 125I. Group B (n = 3): Haemostasis with unlabelled FTAS, subcutaneous injection of 0.1 ml = 60 mu Ci 125I FTAS. Group C (n = 6): Haemostasis with 125I labelled FTAS. Group D (n = 16): treated like Group C. In Groups A - C 125I-elimination in 24 h urine samples was determined with a gamma-scintillation counter. Pairs of animals in Group D were killed after 2, 6, 12 and 24 h and 3, 7, 14 and 21 days. Kidneys were examined under the light and electron microscope and by autoradiography. In animals of Groups B and C two peaks of 125I excretion were observed: one peak within the first 48 h postoperatively which corresponded to the amount of free iodine injected with FTAS (FTAS contains 15% free iodine); a second peak after 120 h which was most probably due to the degradation of FTAS. Fibrinolysis was not observed. FTAS was resorbed mainly by macrophages. The time course of wound healing paralleled that of physiological fibrinogen concentration. Renal parenchymal damage was not observed.