Abstract
Human hyperplastic prostate tissue was homogenised in high ionic strength buffer and the post nuclear homogenate was incubated with 0.8% octyl glucoside and bovine brain lipids. Dialysis of the resulting liposome suspension yielded a preparation in which 5α-reductase was active and stable for at least three weeks and showed an increase in specific activity ( V max±SD=48.9±7.4pmol DHT/mg protein/ml) over that of the starting homogenate ( V max±SD=5.6±1.5pmol DHT/mg protein/min) of 8.7 times.
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