Abstract
A major separation and considerable purification of the follicle-stimulating hormone (FSH) and luteinizing hormone (LH, ICSH) activities present in gonadotrophin concentrates prepared from urine of eunuchs were attained by column chromatography on DEAE-cellulose. The principal FSH fraction was 4.1 times more potent than NIH-FSH-S1, according to the augmentation assay of Steelman and Pohley. Contamination with LH activity was relatively low, 0.0021 XNIH-LH-S1 per mg, according to the ovarian ascorbic acid depletion assay of Parlow. Expressed in terms of the IRP-HMG-(24), the FSH potency of the purified eunuch fraction was 780 U/mg and the LH potency was 6.5 U/mg. The principal eunuch LH fraction, 0.013 times as potent as NIH-LH-S1, was virtually devoid of FSH activity (less than 0.02 times as potent as NIH-FSH-S1 per mg). The ratio of FSH: LH activity in the FSH fraction was at least 1300 times higher than this ratio in the LH fraction. FSH and LH fractions similar to those described above were also prepar...
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