Abstract
1. A DNA-dependent RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase, EC 2.7.7.6) has been purified 90-fold from young etiolated maize seedlings. The enzyme has been partially purified from the supernatant fraction by the standard enzymological techniques of ultracentrifugation, salt fractionation and adsorption chromatography. 2. The RNA polymerase from maize requires the simultaneous presence of 4 ribonucleoside triphosphates for the incorporation of any ribonucleotide into acid-insoluble product. Both native and heat-denatured DNA satisfy the template requirement, whereas RNA does not. 3. The product formed is an oligonucleotide having a base composition complementary with that of the template DNA.
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