Abstract
An extracellular haemolytic activity, produced by the fish pathogen Aeromonas salmonicida, against trout erythrocytes (T-lysin) was partially purified by ammonium sulphate precipitation and gel chromatography. Lysis of trout erythrocytes was found to be due to the combined activity of a caseinase and another factor, apparently membrane-associated, which when separated caused incomplete lysis. Incomplete lysis was also observed when caseinase production was suppressed by the incorporation of ammonium sulphate in the growth medium, or in caseinase-negative mutants. Inhibition of caseinase activity by phenyl methyl sulphonyl fluoride also resulted in the loss of full lytic potential from culture supernates containing T-lysin.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
