Abstract
AbstractThe sex pheromone of female Heterodera schachtii, the sugar beet cyst nematode, was partially isolated using high performance liquid chromatography (HPLC). Female-conditioned medium (FCM), produced by incubating 20 H. schachtii females in 1 ml of ultrapure water, attracted males in a concentration dependent manner. The attractive material was not contained within the female gelatinous matrix and did not attract second stage juveniles (J2). Characterisation of FCM revealed that the attractive material was water soluble and heat stable. Male H. schachtii exhibited stylet thrusting behaviour upon exposure to FCM, and this behaviour was used as a novel indicator of biological activity.
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