Partial Decellularized Scaffold Combined with Autologous Nasal Epithelial Cell Sheet for Tracheal Tissue Engineering.

Luong Huu Dang,Nhi Thao Ngoc Le,Ly Xuan Quang,Yuan Tseng,How Tseng,Shih-Han Hung,Chia-Lang Fang

doi:10.3390/ijms221910322

Abstract

Decellularization has emerged as a potential solution for tracheal replacement. As a fully decellularized graft failed to achieve its purposes, the de-epithelialization partial decellularization protocol appeared to be a promising approach for fabricating scaffolds with preserved mechanical properties and few immune rejection responses after transplantation. Nevertheless, a lack of appropriate concurrent epithelialization treatment can lead to luminal stenosis of the transplant and impede its eventual success. To improve re-epithelialization, autologous nasal epithelial cell sheets generated by our cell sheet engineering platform were utilized in this study under an in vivo rabbit model. The newly created cell sheets have an intact and transplantable appearance, with their specific characteristics of airway epithelial origin being highly expressed upon histological and immunohistochemical analysis. Subsequently, those cell sheets were incorporated with a partially decellularized tracheal graft for autograft transplantation under tracheal partial resection models. The preliminary results two months post operation demonstrated that the transplanted patches appeared to be wholly integrated into the host trachea with adequate healing of the luminal surface, which was confirmed via endoscopic and histologic evaluations. The satisfactory result of this hybrid scaffold protocol could serve as a potential solution for tracheal reconstructions in the future.

Highlights

To date, a number of studies on tracheal replacement have been published [1,2].some obstacles need to be improved before an ideal tracheal substitute becomes a reality
We introduced a new decellularization (PD) protocol that can fabricate a transplantable scaffold with preserved partial decellularization (PD) protocol that can fabricate a transplantable scaffold with viable cartilage cellular components that remain functional and gradually restore the lupreserved viable cartilage cellular components that remain functional and gradually restore minal strength of the tracheal transplant without signs of immune rejection [10]
To evaluate the in vivo performance of applying the cell sheet in preventing the stenosis of the decellularized trachea, a partial resection model was used in New Zealand rabbits

Summary

Introduction

A number of studies on tracheal replacement have been published [1,2]. Some obstacles need to be improved before an ideal tracheal substitute becomes a reality. Some researchers have shifted their attention toward biological tracheal scaffolds derived from decellularized tissues [3]. Decellularization via physical, chemical, enzymatic, or combination methods reduces the immunogenicity of the tissue.

Methods

Results

Discussion

Conclusion