Abstract
Much information about gibberellin (GA) biosynthesis in higher plants has come from studies using cell-free systems prepared from immature seeds (for a recent review, see Graebe1). Cell-free systems from immature seeds of Phaseolus vulgaris catalyze the conversion of GA20 to GA1, GA5, and GA6 by 3β-hydroxylation, 2,3-dehydrogenation, and 2,3-epoxidation of GA20, respectively.2–5 Of these reactions, 3β-hydroxylation is particularly interesting because it has been suggested that GA1 is the active GA in the regulation of internode elongation in maize,6 pea,7 and bean.8 Although the physiological function of GAs in immature seeds is unknown, immature seed is a convenient material for the isolation of the enzyme, 3β-hydroxylase.
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