Abstract
An extracellular alkaline protease of Bacillus circulans MTCC7906 was produced using cotton deoiled meal replacing nitrogen source in the production medium at a pH of 10.5, temperature of 25C in 96 hours of incubation. The enzyme purified by (NH4)2SO4 precipitation and dialysis showed optimum activity at 60C and pH 9.0 with an enzyme concentration of 3.33% of reaction mixture at a casein concentration of 14mg ml-1. SDS PAGE of the crude enzyme revealed it as 24.5 kDa protein. The enzyme was stable at room temperature for 20 days and its activity was enhanced by Ca, Mn, Zn, Co and Ba while inhibited by Hg, Cu, NH4OH and EDTA suggesting it as a metal dependent serine protease. The alkaline protease was compatible with six local powdered detergent brands retaining more than 90% activity.
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