Partial cDNA sequence analysis of the boar proacrosin-binding protein

Abstract

A partial cDNA sequence of the boar 28, 29-kDa proacrosin-binding protein was determined. The desired sequence was amplified and obtained by RT-PCR followed by nested PCR. The oligonucleotide primers for RT-PCR were designed by deduction from the internal amino acid sequence of the boar proacrosin-binding protein. An approximately 1-kbp DNA fragment was analysed by agarose gel electrophoresis, in which the boar testicular total RNA or poly(A)+ RNA was used as a template for RT-PCR followed by nested PCR. This PCR product was inserted into a TA vector. The DNA sequence of the inserted PCR product was then determined by the chain termination method.