Partial biological characteristics and genomic analysis of Vibrio cholerae typing phage VP2

Abstract

Objective This study aimed to further understand the genetic information of VP2 by analyzing the whole genome of Vibrio cholerae typing phage VP2, and analyzing the protein of mature particles in combination with biological characteristics. Methods The morphology of phage VP2 was observed under electron microscope, and some of its biological characteristics were determined. Online RAST (https://rast.nmpdr.org/) was used to predict and annotate the encoding genes of the phage VP2 genome, and the proteins in the mature VP2 particles were analyzed by mass spectrometry. Phage genome average nucleotide consistency (ANI, business, nucleotide identity) was analysis by pyani (https://github.com/widdowquinn/pyani). Results Phage VP2 was a typical 20-hedron short-tailed phage, the optimal MOI is 0.01. One step growth curve showed that incubation period is about 60 minutes, 60 minutes to 120 minutes for the outbreak period, after 120 minutes for the stable period. Forty-nine open reading frames (ORFs) were predicted. Protein spectrum analysis of mature VP2 particles showed that 34 proteins corresponded to the predicted genes. ANI cluster analysis showed that ANI was the highest in VP2 and vibro phage CJY. Conclusion The morphological and genomic characteristics of typing phage VP2 were identified, which laid a foundation for the further study of homologous phage and its interaction with Vibrio cholerae.