Abstract
Feverfew (Tanacetum parthenium) is one of the most important medicinal plants with different pharmacologic properties, such as anti-inflammatory, cardiotonic, antitumor and antiangiogenic activities. Parthenolide (PN) is a main bioactive molecule in feverfew which belongs to sesquiterpene lactone compounds. Currently, the plant cell suspension has been used as a useful method to produce secondary metabolites (SMs) components. Meanwhile, the elicitor application is an effective strategy to induce the production of SMs in plants. The present study was conducted as two different experiments in cell suspension of feverfew. In the first experiment, the effects of explant (shoot and root), hormone (TDZ + NAA and TDZ + 2. 4-D) on cell dry weight for one month were investigated. In the second experiment, the effect of elicitor (namely, MJ, YE and Ag+) and the hormones after 24, 48 and 72 h on PN content was assessed. The result of the first experiment revealed that the simple effects and the interaction of hormone × explant were significant (P < 0.01) for cell dry weight. Growth rate analysis showed that shoot-derived cell suspension in 1 mg L−1 NAA + 0.5 mg L−1 TDZ treatment had the highest amount of cell dry weight 14 days after the culture. According to the second experiment, the highest PN content was obtained in cell suspension containing 0.5 mg L−1 2, 4-D + 0.1 mg L−1 TDZ with application of the YE + MJ elicitor after 48 h. The cell suspension treatment with each of the elicitors had a positive effect on the PN production. In conclusion, the application of combined elicitors in feverfew cell suspension culture can be used as an efficient tool for large-scale PN production.
Highlights
Feverfew (Tanacetum parthenium) is one of the most important medicinal plants with different pharmacological and therapeutic properties (Pourianezhad et al 2016)
We have previously investigated the effects of various elicitors including yeast extract (YE) (2.5 mg L−1), methyl jasmonate (MJ) (100 μM), Ag+ (100 μM), YE + MJ, Ag+ + MJ, YE + Ag+ and YE + MJ + Ag+ on the production of PN in feverfew hairy root culture (Pourianezhad et al 2019)
Based on the mean comparison results, the highest amount of cell dry weight was obtained from shoot-derived cell suspension in 1 mg L−1 NAA + 0.5 mg L−1 TDZ treatment, which was significantly different from the other treatments
Summary
Feverfew (Tanacetum parthenium) is one of the most important medicinal plants with different pharmacological and therapeutic properties (Pourianezhad et al 2016). It is a perennial plant of Asteraceae family. The plant is native to Kazakhstan, Central Asia and Mediterranean region, but it can be found in different parts of Europe, Asia and America (Arabasi and Bayram 2005; Farzaneh et al 2002). The plant has been used to treat a wide range of diseases such as menstrual disorders, toothache, migraine, asthma, fever, stomach ache, arthritis, insect bites and helminthiasis (Maggi 2019; Sadat-Hosseini et al 2017).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
