Abstract
It is known that serum from allergy and asthma patients contains elevated levels of immunoglobulin E. This research reports use of a simple ELISA test for measuring levels of endogenous proteins, including immunoglobulin E, nerve growth factor, myoglobin, and insulin in non-invasively collected saliva. The elevated immunoglobulin E is believed to play a role in mediating symptoms of allergy/asthma, type 2 diabetes, and autoimmune diseases. High levels of immunoglobulin E were associated with increased glucose concentration and disruption of the regulation of levels of other endogenous proteins. Saliva from normal people and patients suffering from type 2 diabetes and various other disorders, such as autoimmune diseases, were assayed for immunoglobulin E, nerve growth factor, myoglobin, and insulin using a simple ELISA test employing the respective anti-sera. It was observed that saliva specimens from individuals with type 2 diabetes and various autoimmune diseases contained elevated levels of immunoglobulin E, nerve growth factor, myoglobin, and insulin in comparison with saliva samples from normal control individuals.
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