Abstract
Aims To compare the sensitivity of ELISA for parietal cell antibody to gastric H/K ATPase with immunofluorescence Methods A retrospective set of sera selected for positivity or negativity for intrinsic factor antibody and a set of prospective sera were tested for reactivity to gastric H/K ATPase by ELISA and for immunofluorescence to gastric parietal cells. Results 87 of 138 sera reacted with gastric H/K ATPase by ELI-SA and 62 with gastric parietal cells by immunofluorescencence giving the ELISA an increased sensitivity of 32% over immuno-fluorescence. Only three sera weakly positive by immunofluores-cence were negative by ELISA. Similar results were obtained with another ELISA kit. All 16 sera positive by ELISA and negative by immunofluorescence immunoblotted 100 kDa and 60–90 kDa proteins of gastric membranes consistent with the mw respectively of the α and β subunits of the gastric H/K ATPase. Forty-six of 53 (87%) intrinsic factor positive sera also reacted to the gastric H/K ATPase. Similar results were obtained with the prospective 161 serum samples. Conclusions ELISA for gastric H/K ATPase is a more sensitiv e test than immunofluorescence for detecting parietal cell antibody and segregates with intrinsic factor antibody. The ELISA can thus be applied instead of immunofluorescence for diagnostic screening for parietal cell antibody.
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