Abstract
Aerial basidia were produced after 3 wk on malt extract or sporulation agar but were more prominent on the latter medium. Aerial basidia produced basipetal chains consisting of up to 15 spores per chain. Single-basidiospore isolates obtained by micromanipulation produced yeast cells typical of C. neoformans in vitro as well as in vivo and were pathogenic for mice. The assimilation pattern of the single-basidiospore isolates was also that of C. neoformans. I am indebted to Dr. Lindsay S. Olive, University of North Carolina, Chapel Hill, North Carolina, for his critical reading of the descriptions and Miss Edith Cash, Nineveh, New York, for preparation of the Latin diagnoses.
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