Abstract
Background Parenteral nutrition (PN) increases post-trauma pneumonia versus enteral feeding. PN impairs murine immunoglobulin A (IgA) airway defenses and abrogates a normal IgA increase following injury. This work investigates the effect of type/route of nutrition on lung IgA and its transport protein, polymeric immunoglobulin receptor (pIgR), after injury. Methods Catheterized mice were randomized to Chow or PN for 5 days and sacrificed without injury (Chow: n = 12; PN n = 11), or 8 hours after laparotomy + neck incisions (Chow-injury: n = 11, PN-injury: n = 13). Bronchoalveolar lavage (BAL) and lung IgA levels were analyzed by enzyme-linked immunosorbent assay (ELISA) and lung pIgR by Western blot. Results BAL IgA levels increased in Chow-injury versus PN-injury ( P <.01) with no differences in pIgR. PN-injury tissue IgA levels decreased versus Chow ( P <.01), Chow-injury ( P <.01), and PN ( P <.05). Conclusions PN impairs the airway IgA response to injury but not due to impaired IgA transport capacity/pIgR level.
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