Parathyroid Hormone-Responsive Clonal Cell Lines from Mouse Growth Cartilage

Abstract

The major target organs for parathyroid hormone (PTH) are the bone and kidney. PTH regulates or influences metabolism of calcium and phosphate in these tissues and its action is presumably mediated by 3’, 5’-cyclic AMP (cAMP) (Rosenblatt, 1982). For investigation of the role of cAMP in mediating the action of PTH, several PTH-responsive clonal cell lines have been established from bone (Aubin et al., 1982; Nakatani et al., 1984) and osteosarcoma (Majeska et al., 1980; Partridge et al., 1983). However, no PTH-responsive cell line has yet been established from cartilage, which is another target organ for PTH (Takigawa et al., 1979; 1980; Kawashima et al., 1980). We have shown that PTH increases the intracellular cAMP level in rabbit costal chondrocytes grown in primary culture (Takigawa et al., 1981) but that the cells lose the abilities to proliferate and to respond to PTH during successive passages (Takigawa et al., 1987). This paper reports the establishment from mouse growth cartilage (GC) of a cell line, and derivative clonal lines that respond to PTH.