Abstract
Although secondary hyperparathyroidism is a common complication of chronic renal failure, few studies have examined the characteristics of parathyroid hormone (PTH) binding to the kidney or the regulation of the PTH receptor in chronic renal disease. In this study we measured PTH binding to the PTH-1 receptor in renal cortical membranes from normal rats and from rats with experimentally induced chronic renal failure. In normal rats, analysis of saturation binding experiments using <sup>125</sup>I PTH-related peptide (chicken, cPTHrP) revealed apparent K<sub>d</sub> and B<sub>max</sub> values of 1.16 ± 0.14 nmol/l and 338 ± 22.7 fmol/mg, respectively. Three weeks following induction of renal failure there was no change in the affinity of the PTH-1 receptor (K<sub>d</sub> = 1.51 ± 0.24 nmol/l) but the B<sub>max</sub> was reduced by 45% (183 ± 32.5). In normal rats which had undergone thyroparathyroidectomy, the K<sub>d</sub> was unchanged (1.17 ± 0.09) while the B<sub>max</sub> increased to 459 ± 31 fmol/mg. We conclude that chronic renal failure is accompanied by a downregulation of renal PTH-1 receptors.
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