Abstract
Received at: 30/3/2014 Accepted: 16/4/2014 The present study was carried out to investigate the prevalence of fascioliasis among 100 cattle and buffaloes of different age and sex in Assiut Governorate. Samples were examined by microscopic examination, Agar Gel Diffusion Test AGDT and ELISA techniques to detect Fasciola species eggs and antibodies, respectively. Prevalence of Fasciola was 8% by microscopic examination, 32% by AGDT and 60.86% by ELISA test. Cattle showed higher prevalence of infection 61.4% than buffaloes 29.4%. Female cattle and buffaloes were more susceptible to the infection 64.55% than male 23.8%. Fasciola was more frequently recorded in 2-4 years old cattle and buffaloes 76% followed by more than 4 years 46.7% then less than 2 years of age 20%. All positive results with fecal egg examination and AGDT were also positive with ELISA. Serological examination by AGDT and ELISA confirms the microscopical examination. The results of this study revealed that the agar gel diffusion test and ELISA could become a useful tools to diagnose fascioliasis in cattle and buffaloes.
Highlights
Haridy et al (2002); Mas-Coma, et al (2005) and Soliman (2008) mentioned that fascioliasis is a serious infectious parasitic disease infecting domestic ruminants and humans, tops all the zoonotic helminthes worldwide
Out of the 100 cattle and buffaloes examined for Fasciola species 8% (8/100) were positive by microscopic examination (Figure 5), 32% (32/100) by Agar Gel Diffusion Test (AGDT)
While antibody detection by Enzyme Linked Immunosorbent Assay (ELISA) tests resulting in a prevalence of fascioliasis was 60.87% (56/92) by ELISA (Table 1)
Summary
Haridy et al (2002); Mas-Coma, et al (2005) and Soliman (2008) mentioned that fascioliasis is a serious infectious parasitic disease infecting domestic ruminants and humans, tops all the zoonotic helminthes worldwide. Serological methods can test a large number of sera at a time and detect infection earlier than fecal egg examination.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
