Abstract
This study aimed to find out the parasitemia of DDY white mice infected with T. evansi of Pidie and Pemalang isolates obtained from Bbalitvet Bogor. A total of 12 mice were divided into 3 treatment groups. Group 1 (K1) without any treatment, group 2 (K2) was inoculated with 104 T. evansi Pidie isolate, and group 3 (K3) was inoculated with 104 T. evansi Pemalang isolate. Parasitemia examination was carried out every two days and the level of parasitemia was observed as well. Parasitemia examination was conducted until all mice died. Parasitemia of mice infected with Pidie isolate was characterized by rapid rise of parasitemia in blood (107-108/mL of blood) in a short time (2-4 days) since first parasitemia was detected and followed by death at day 4. The parasitemia of mice infected with Pemalang isolate increased in the blood (108-109/mL of blood) on day 4 and maintained for a few more days and then fluctuated for a few more days before the animal was dead. In conclusion, there was the difference in parasitemia level between Pidie and Pemalang isolates. Key words: Trypanosoma evansi, parasitemia, Pidie isolate, Pemalang isolate
Highlights
This study aimed to find out the parasitemia of DDY white mice infected with T. evansi of Pidie and Pemalang isolates obtained from Bbalitvet Bogor
Preliminary evaluation of diagnostic tests using horses experimentally infected with Trypanosoma evansi
Summary
Sebanyak 12 ekor mencit putih (galur DDY) jantan digunakan dalam penelitian ini dibagi ke dalam 3 kelompok perlakuan, masing-masing terdiri atas 4 ekor. Kelompok kedua (K2) diinokulasikan 104 T. evansi isolat Pidie. Kelompok ketiga (K3) diinokulasikan 104 T. evansi isolat Pemalang. Mencit yang digunakan dalam penelitian ini diberi tanda identitas secara individual dengan pewarnaan asam pikrat dan dipelihara dalam 3 buah kandang terpisah. Setelah diinfeksi, masing-masing mencit diperiksa parasitemianya setiap dua hari sekali. Propagasi Isolat Trypanosoma evansi Masing masing isolat diremajakan dan diperbanyak pada mencit. Apabila parasitemia telah mencapai 107108 Trypanosoma/ml darah, maka isolat T. evansi tersebut sudah dapat digunakan dalam penelitian (Subekti et al, 2013)
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