Abstract
Protoplasts of multiauxotrophic strains of Nectria haematococca (the perfect form of Fusarium solani) were fused and grown on different selective media. Following protoplast fusion, rapidly growing heterokaryons were formed at high frequency (about 1%). By collecting uninucleate microconidia from these heterokaryons, it was possible to isolate a few colonies with new combinations of the parental markers. On some selective media, non-heterokaryotic fusion products, easily distinguishable from heterokaryons by their growth characteristics, were detected at low frequency. These colonies were either stable haploid recombinants or unstable ‘hybrids’ of unknown ploidy. Genetic analysis of ‘hybrids’ in which six factors were heterozygous provided evidence for nuclear fusion events. These ‘hybrid’ colonies spontaneously segregated a large number of haploid recombinants, allowing the analysis of linkage relationships between markers. The detection of a mitotic linkage between two markers.which appeared unlinked following meiosis, demonstrates that mitotic mapping may be an important complement to meiotic analysis for mapping the chromosomes of Nectria haematococca.
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