Paraphernalia of Growth Regulators During In Vitro micro-Propagation of Grapevine (Vitisvinifera L.) from Shoot Tips and Nodal Segments

Abstract

As grapevine (Vitis vinifera L.) is rarely produced in Bangladesh because of unavailability of improved varieties, so this study was designed to solve this problem through evaluating the effects of hormonal combination for the duration of in vitro micro propagation of grapevine (Vitis vinifera L.) from shoot tips and nodal segments. Firstly, surface sterilization process was carried out by using HgCl2 (mercuric chlorite) at 0.1% for 3 min and best result was found. During establishment stage, explants were cultured on MS (Murashige and Skoog) basal medium supplemented with BAP (6-benzylamino purine) 0.5, 1.0 and 2.0 mg/l and NAA (β-naphthalene acetic acid). 0.1mg/l where MS+ BAP 1.0 mg/l + NAA 0.1mg/l displayed best potential result. During shoot multiplication stage, BAP 2.0 and 3.0 mg/l and NAA 0.1, 0.2 and 0.3 mg/l and their combination were used and highest number of proliferated shoots was obtained from MS+ BAP 3.0 mg/l + NAA 0.2 mg/l. For rooting stage, NAA 0.5 and 1.0 mg/l and IBA (Indol-3-butyric acid) 0.5, 1.0 and 1.5 mg/l were used and tested. The highest rooting percentage, number of roots per shoot and root length found in MS+ 0.5 mg/l NAA + IBA 1.0 mg/l. Finally, neo-formed plantlets were transferred into pots containing peat moss and sand (1:1 v/v) and potential growth of these plantlets in environment indicates that through using the adequate amount of hormonal combination could give a better solution for the improvement and availability of grapevine (Vitis vinifera L.) for Bangladeshi farmers.