Abstract
The present research work is about the production and process optimization of pectinase enzyme using substrate Ficus religiosa leaves by Aspergillus awamori in solid state fermentation. Pectinase having various applications in food industry such as fruit juice extraction, coffee and tea fermentation, textile, paper and pulp industries and in waste-water treatment. Solid-state fermentation is described as a process that take place on a non-soluble material that performs both as support and a source of nutrients, with a reduced among of water, under the action of fermenting agent. Parametric evaluations like time, temperature, size of inoculum, pH and moisture content were optimized to induce the high yield of pectinase. The increased level of pectinase enzyme production was detected at time 72 hrs, temperature 30 °C, optimum inoculum level was 50% v/w, pH 5 and 60 % v/w moisture content of the substrate were best for the maximum production of pectinase in solid-state condition. A remarkable enzyme production was enhanced and recorded when the basal medium was supplemented with carbon (4% glucose) and nitrogen (ammonium sulphate, 0.4%) sources.
Highlights
Pectins are high molecular weight acid polysaccharides, predominantly made up of α-(1→ 4) linked D-galacturonicacid remainder with a small number of rhamnose remains in the main chain and arabinose, galactose and xylose on its side chain [1,2,3,4]
Microorganism Aspergillus awamori was used for the production of pectinase enzyme using Ficus religiosa leaves as substrate
Preparation of inoculum Streaking is done on pure potato dextrose agar slants from the old culture of Aspergillus awamori and streaked agar slants are incubated at 30 oC for 3 days
Summary
Pectins are high molecular weight acid polysaccharides, predominantly made up of α-(1→ 4) linked D-galacturonicacid remainder with a small number of rhamnose remains in the main chain and arabinose, galactose and xylose on its side chain [1,2,3,4]. Pectinases are enzymes that break down pectin which is polysaccharide that available in plant cell walls. Pectinase enzymes are mostly applied in the process of degradation of plant materials, including speeding up the extraction of fruit juice from fruit, like apples and sapota etc. Solid-state fermentation [SSF] is a process that take place on a non-soluble material that performs both as support and a source of nutrients, with a reduced among of water, under the action of fermenting agent [6]. Keep in view of the importance pectinase enzyme and the advantage of SSF, the present investigation proposed to study the production and parametric optimization of pectinase enzyme from cheaply and abundantly available raw material
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
