Abstract
This study reveals the selection of a yeast strain, possessing inulinase activity and finding the optimal conditions of cultivation. Intra- and extracellular activity assay was performed after cultivation on media, containing inulin as a sole source of carbon. Optimization of the cultivation conditions was carried out for establishing the favorable conditions for biosynthesis of inulinase. Modifying the physicochemical and nutritional parameters of a cultivation process lead to major improvement of the enzyme activity. Highest intra- and extracellular inulinase activity was registered when 1.5% inulin was used, 5 % inoculum, temperature 28°C, pH=6.5 and agitation of 200rpm. The selected strain Kluyveromyces sp. C showed higher values for the intracellular inulinase activity, making it suitable for immobilization and further use. Key words: Kluyveromyces sp., inulinase activity, parameters optimization
Highlights
In order to meet Guinea's energy needs, significantly reduce local pollution and the greenhouse effect, and preserve the environment, it is necessary to explore and develop new energy sources such as biogas
We have carried out three experiments of anaerobic digestion each one lasting 45 days at mesophilic temperature. 28.4 liters have been obtained from droppings and 22.6 liters from cow dung and 38.7 liters from co-digestion of the previous two wastes
The following physico-chemical characteristcs were observed for cow dung: humidity 43%, dry matter 20.83%, organic matter 57%, density 625kg/m3, carbon content 31%, nitrogen content 1.46%, nitrogen-carbon ratio 21/30
Summary
In order to meet Guinea's energy needs, significantly reduce local pollution and the greenhouse effect, and preserve the environment, it is necessary to explore and develop new energy sources such as biogas. This widely available, inexpensive and non-polluting renewable energy can be used to complement so-called nonrenewable fossil fuels [1]. Organic wastes may be turned into a source of wealth in remote rural areas thanks to anaerobic digestion. Animal wastes, thanks to the organic matter they contain, are a guarantee for soil fertility. The gaseous emissions from these effluents are methane, CH!, ammonia, NH", hydrogen sulphide, H#S, carbon dioxide, CO#
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