Abstract
Abstract Mitochondrial H + (Mg 2+ )-ATPase (EC 3.6.1.3) activity of Neurospora crassa has been studied during conidial germination in the presence and absence of 2,4-dinitrophenol and dicoumarol. The activity was optimal at pH 8.5–9.0 and was inhibited (80–90%) by oligomycin, N , N '-dicyclo-hexylcarbodiimide and azide. The enzyme activity already present in mitochondria isolated from dry- and wet-harvested conidia did not change significantly during the 2.5 h pregermination at 25°C (20% outgrowth), but increased sharply up to 7.5 h corresponding to the end of germination period and remained steady until the end of log phase (15 h). In uncoupler-treated conidia, the stimulation of germ tube outgrowth at 2.5 h was found to parallel the activation (40%) of mitochondrial ATPase.
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