Abstract
Drought is a complicated abiotic stress factor with severe effects on rice growth and production. Weedy rice is a valuable genetic resource that possesses a strong capacity for drought tolerance, cold tolerance, and salt tolerance, and is an excellent material for studying rice tolerance. Here, according to comprehensive tolerance to drought index D, accession WR16 was selected based on strong drought tolerance among 133 studied weedy red rice germplasms. WR16 was compared with Oryza sativa ssp. Japonica. cv. IAPAR-9, a reference genotype originating from Brazil. In addition, accession WR24 was classified as moderately tolerant to drought accessions. Transcriptomic and proteomic analyses were combined to identify 38 co-upregulated proteins related to drought tolerance, and targeted parallel reaction monitoring (PRM) was used to precisely quantify and verify nine proteins in the complex backgrounds. Result showed that six proteins were significantly (Fisher's exact P value < 0.05) related to drought tolerance in accessions WR16 and WR24. Among them, OS09T0478300-01, OS09T0530300-01, and OS01T0800500-01 formed a combined defense system to respond to drought stress in weedy rice. Results of these studies provide comprehensive information for precisely identifying and verifying tolerance to drought proteins and lay a solid theoretical foundation for research on drought tolerance mechanisms.
Highlights
Drought is a complicated abiotic stress factor with severe effects on rice growth and production
Identifying drought tolerance germplasm in weedy rice accessions through new, precise methods is critical for further study of the genetic and molecular mechanism associated with drought tolerant traits
The identification experiment of drought tolerance for 133 weedy rice accessions was constructed at the Nanbin farm in Sanya city of Hainan province, China, from January 25 to March 22, 2017, total consecutive 57 days, it is not rain in the region (data from the weather forecast of 2017 (Tables S1, S2)
Summary
Drought is a complicated abiotic stress factor with severe effects on rice growth and production. Transcriptomic and proteomic analyses were combined to identify 38 co-upregulated proteins related to drought tolerance, and targeted parallel reaction monitoring (PRM) was used to precisely quantify and verify nine proteins in the complex backgrounds. OS09T0478300-01, OS09T0530300-01, and OS01T0800500-01 formed a combined defense system to respond to drought stress in weedy rice Results of these studies provide comprehensive information for precisely identifying and verifying tolerance to drought proteins and lay a solid theoretical foundation for research on drought tolerance mechanisms. Combining omics analysis will greatly shorten the time of identifying germplasms and genes and accelerate the breeding process of tolerance to drought accession[3] These genetic resources function directly in facilitating discovery of functional variants and enhancing our ability to control and utilize weedy rice for rice improvement. Proteomic analysis by iTRAQ-PRM provides novel insights into the molecular mechanism response of plant to B. tabaci in Pepper[6]
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