Abstract
The Fresnel incoherent correlation holography (FINCH) method is applicable to various techniques of imaging, including fluorescence microscopy. Recently, a FINCH configuration capable of optical sectioning, using a scanning phase pinhole, has been suggested [Optica 1, 70 (2014)]. This capability is highly important in situations that demand the suppression of out-of-focus information from the hologram reconstruction of a specific plane of interest, such as the imaging of thick samples in biology. In this study, parallel-mode scanning using multiple phase pinholes is suggested as a means to shorten the acquisition time in an optical sectioning FINCH configuration. The parallel-mode scanning is enabled through a phase-shifting procedure that extracts the mixed term of two out of three interfering beams.
Highlights
Fresnel incoherent correlation holography (FINCH) has been introduced almost a decade ago as a method for recording Fresnel holograms under spatially incoherent illumination [1]
Utilizing modern electro-optical devices, such as spatial light modulators (SLMs) and digital image sensors, together with phase-shifting techniques [2], FINCH has given a new birth to the classical concept of self-interference-based incoherent holography [3,4] into the era of digital holography
FINCH offers enhanced lateral resolution, in the sense that while the cut-off frequency of its modulation transfer function (MTF) is the same as the cut-off frequency of an incoherent imaging system of similar numerical aperture, the shape of the MTF is similar to that of a coherent imaging system, which is uniform throughout the passed frequencies, and does not decay non-zero frequencies like in an incoherent imaging system [13]
Summary
Fresnel incoherent correlation holography (FINCH) has been introduced almost a decade ago as a method for recording Fresnel holograms under spatially incoherent illumination [1]. A Nipkow spinning disk was inserted to provide optical sectioning before light even reaches the actual holographic interferometer (i.e., before any splitting of waves occurs) In a sense, this may be considered an off the shelf, readily available solution to optical sectioning, but it is a successful testament that a confocal FINCH system can be made competitive with conventional confocal systems in terms of lateral resolution, sectioning performance and applicability. A major drawback of these two solutions for optical sectioning using FINCH is the requirement of a scanning procedure In the latter case [24], the scanning is mechanical but is rather quick due to the use of the Nipkow spinning disk. Unlike [22], they are not suitable for fluorescence imaging [30] or situations in which the user does not have control over the illumination
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