Abstract
We have studied the antibody binding capacity of mouse H–2 antigens after fixation with paraformaldehyde. Spleen cells and erythrocyte stroma fixed for one hour in 0.9% paraformaldehyde were found to bind anti‐H–2 antibodies to essentially the same extent as fresh material. Mouse macrophages fixed in this way were shown to be unable to pinocytose peroxidase. The fixation procedure described here should be useful in studies of the amount and distribution of H–2 antigens on mouse cells.
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