Abstract
1. The Parafalg technique is one in which a wholly basic dye (Pararosanalme chloride) is the primary stain and light green (SF, yellowish) is used as a secondary and supplementary stain. 2. A trichromatic picture in red, purple and blue is produced, these colours corresponding to high, medium and low acidity, respectively. 3. Details of the technique are given, including a method of blocking basic tissues against the acid light green supplementary stain. 4. The significance of the results found are discussed and it is shown that by the use of Palg and Parafalg methods a 16-fold classification of tissue elements is possible. 5. The staining of DNA and RNA loci is discussed briefly. This work was carried out with the aid of special technical assistance provided by the Medical Research Council of Ireland, for which we express our thanks.
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