Abstract
Chromophore identification in biological samples often requires the physical separation of the compounds, which can be difficult. Although there are several advantages to hyphenated spectroscopic techniques for identification of substances, complex mixtures of chromophores presenting overlapped spectra cannot be identified directly through this method. This work presents an application of chemometrics to compound identification in biological samples by a spectroscopic hyphenated technique using a curve resolution method. The PARAllel FACtor analysis model (PARAFAC), which has no rotational indeterminacy, was used for curve resolution of excitation-emission spectra of human dental tartars. PARAFAC was applied under constraints (i.e., unimodality and non-negativity) and evaluated with a validation procedure. The resolved profiles are porphyrinic-like spectra presenting excitation band maxima at 407, 416, and 431 nm in the Soret band region (390–440 nm) of these substances.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
