Paradigm of Time-sequence Development of the Intestine of Suckling Piglets with Microarray.

Yunzi Sun,Keying Zhang,Daiwen Chen,Xijian Chen,Bing Yu

doi:10.5713/ajas.2012.12004

Yunzi Sun, Keying Zhang + Show 3 more

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https://doi.org/10.5713/ajas.2012.12004

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Abstract

The interaction of the genes involved in intestinal development is the molecular basis of the regulatory mechanisms of intestinal development. The objective of this study was to identify the significant pathways and key genes that regulate intestinal development in Landrace piglets, and elucidate their rules of operation. The differential expression of genes related to intestinal development during suckling time was investigated using a porcine genome array. Time sequence profiles were analyzed for the differentially expressed genes to obtain significant expression profiles. Subsequently, the most significant profiles were assayed using Gene Ontology categories, pathway analysis, network analysis, and analysis of gene co-expression to unveil the main biological processes, the significant pathways, and the effective genes, respectively. In addition, quantitative real-time PCR was carried out to verify the reliability of the results of the analysis of the array. The results showed that more than 8000 differential expression transcripts were identified using microarray technology. Among the 30 significant obtained model profiles, profiles 66 and 13 were the most significant. Analysis of profiles 66 and 13 indicated that they were mainly involved in immunity, metabolism, and cell division or proliferation. Among the most effective genes in these two profiles, CN161469, which is similar to methylcrotonoyl-Coenzyme A carboxylase 2 (beta), and U89949.1, which encodes a folate binding protein, had a crucial influence on the co-expression network.

Highlights

The pig (Sus scrofa) has been widely used as a model for studying the development of human organs and disease progression, owing to the similarity in size and physiology of the two species (Butler et al, 2007; Lunney, 2007; Ojeda et al, 2008)
We aimed using microarray technology to identify the significant pathways and key genes that regulate the intestinal development of Landrace piglets
Results of the analysis of Gene Ontology (GO) categories for profile 66 implied that the main enriched GO categories were focused on control checkpoints for cell size, cell wall biogenesis, deoxyribonucleotide catabolic processes, fungal-type cell wall biogenesis, fungal-type cell wall organization and biogenesis, lymphocyte chemotaxis, M phase-specific microtubule processes, membrane depolarization, microvillus biogenesis, mitochondrial depolarization, mitotic metaphase, etc. during growth from 0 to 21 d (Table 3)

Summary

INTRODUCTION

The pig (Sus scrofa) has been widely used as a model for studying the development of human organs and disease progression, owing to the similarity in size and physiology of the two species (Butler et al, 2007; Lunney, 2007; Ojeda et al, 2008). Microarray technologies have enabled researchers to explore the dynamics of transcription on a genome-wide scale (Young, 2000; Jiang and Deyholos, 2006; Zhang et al, 2008), and they provide a systematic insight into the crucial factors that control development. They can be used to elucidate the mechanisms of intestinal development at a molecular level. We aimed using microarray technology to identify the significant pathways and key genes that regulate the intestinal development of Landrace piglets

MATERIALS AND METHODS

DISCUSSION

Method