Abstract
Viral and bacterial agents causing bovine respiratory disease complex (BRDC) were examined over a five-year period (2015-2019). Thirty-eight farms of beef and dairy cattle from Central Serbia which showed symptoms of BRDC were observed. A total of 118 nasal swab specimens collected from diseased bullocks (n=55), cows (n=18) and calves (n=45) were chosen for the isolation of bacteria and identification by aerobic cultivation. The most commonly isolated bacterial pathogen was Pasteurella multocida (72.88% positive samples). Disk-diffusion method (antibiogram) showed that Amoxiclav and Enrofloxacin were the most efficient antibiotics against Pasteurella multocida isolates (66.67% and 54.17%, respectively). From all examined samples, using the Real Time RT-PCR and PCR methods, we determined the genome sequences of bovine respiratory syncytial virus (BRSV-34.78% of tested samples (8/23), bovine viral diarrhea virus (BVDV, in 34.37% (11/32) and Mycoplasma spp. in 33.33% (8/24). The genome of BoHV-1 virus was not detected in any of the 19 samples tested by Real Time PCR method.
Highlights
Bovine respiratory disease complex (BRDC) is one of the most significant causes of cattle morbidity and mortality, both in beef and dairy cattle, causing enormous costs for the cattle industry, which are reflected in reduced production, increased labor costs and reduced carcass value, regardless of the use of modern vaccines and antibiotics (Irsik et al, 2006; Gershwin et al, 2015)
From all examined samples, using the Real Time RT-PCR and PCR methods, we determined the genome sequences of bovine respiratory syncytial virus (BRSV– 34.78% of tested samples (8/23), bovine viral diarrhea virus (BVDV, in 34.37% (11/32) and Mycoplasma spp. in 33.33% (8/24)
Nasal swabs were sampled at the request of the owners, who reported in anamnestic data that the diseased animals showed symptoms of respiratory disease, which seemed to justify the suspicion of BRDC
Summary
BRDC is one of the most significant causes of cattle morbidity and mortality, both in beef and dairy cattle, causing enormous costs for the cattle industry, which are reflected in reduced production, increased labor costs and reduced carcass value, regardless of the use of modern vaccines and antibiotics (Irsik et al, 2006; Gershwin et al, 2015). BRDC is caused by one or more of several viruses: bovine respiratory syncytial virus (BRSV), bovine herpes type 1 (BoHV-1) known as infectious bovine rhinotracheitis (IBR)/infectious pustular vulvovaginitis (IPV), and bovine viral diarrhea virus (BVDV), which predispose animals to coinfection with one or more bacteria, including Pasteurella multocida (P. multocida), Mannheimia haemolytica (M. haemolytica), Mycoplasma bovis (M. bovis), and Histophilussomni (Gershwin et al., 2015). Viral agents such as BRSV and bovine parainfluenza virus 3 (BPIV-3) are designated as the most common challengers of BRDC. The results of the above mentioned research serve as a basis for the development of innovative therapeutic strategies and for the selection of cattle with enhanced resistance to BRDC (Behura et al, 2017)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
